LINCOLN MEMORIAL UNIVERSITY
SCHOOL OF NURSING AND ALLIED
HEALTH
DEPARTMENT OF VETERINARY
SCIENCE
3
credit hours Spring Semester 2003
Prerequisites: All previous Vet Tech courses.
Textbook: Laboratory Procedures for Animal
Health Technicians. 4th ed. Edited by
Paul W. Pratt, DVM.
Methods of Instructions:
Lecture; slide
presentation, lectures given at UT pertaining to
Hematology, and
“hands-on” experience in lab sessions.
Course Objectives:
The student will be
able to perform various laboratory procedures
pertaining to
hematology and will have a workable knowledge of the
theories involved.
Course Description:
This course will
familiarize students to the laboratory equipment used
in veterinary
clinics and hospitals. The course
includes a complete
description of
laboratory procedures involving hematology, which
the student may come in contact with
in veterinary practice.
Course Requirements:
Student must attend
lectures and complete all reading assignments.
Units of Instruction:
Composition of
Blood
a. liquid portion of blood
b. color variations (Icterus index)
c. function
Red Blood Cells
a. physical description
b. function
c. composition
d. life span
e. erythron
f. production of cells
g. determining functional status of the RBC/erythron
h. erythrocyte count
i. hemoglobin
j. abnormal morphology if RBC’s
k. abnormalities of color
l. pathological conditions
Leukocytes
a. functions (general)
b. special properties
c. two basic groups
Methods of Evaluation:
There will be 5 tests and a comprehensive final. All are counted equally. Articles and
homework will be average as one test grade. Your lab grade will be based on
performance and a lab final. Lab is 20% of your grade. More than one absence in lab
constitutes a drop in a letter grade for each absence. Lab exams will not be made up.
A. Plasma/Serum
1) liquid portion of blood
2) color variations (Icterus Index)
3) function
B. RBC’s
1. physical description
2. function
3. composition
4. life span
5. erythron
6. production of cells
7. determining functional status of the RBC/erythron
a) PCV
1. microhematocrit
2. Wintrobe method
8. erythrocyte count
a) hemacytometer + pipette
b) hemacytometer + unopette
c) cell counter
9. hemoglobin
10. abnormal morphology of RBC’s
a) leptocytes
b) crenation
c) acanthocyte
11. abnormalities of color
a) hypochromasia
b) polychromasia
12. pathological conditions
a)anemias
C. Leukocytes
1) functions (general)
2) special properties
a) diapedisis
b) ambeoid movement
c) phagocytosis
d) chemotoxis
3) Two basic groups
a) polymorphonuclear-granulocytes
1. neutrophils
a. segmented
b. band/stab
2. eosinophils
3. basophils
b) mononuclear-agranulocytes
1. lymphocytes
2. monocytes
4. specific functions
a) neutrophils
b) eosinophils
c) lymphocytes
d) monocytes
e) basophils
5. origin of WBCs
6. maturation stages of monocytes
7. maturation stages of lymphocytes
8. WBC determinations
a)hemacytometer with pipette
b)hemacytometer with unopette
c) electronic cell counter
9. differential blood smear
TEST 3 10. Interpretations
a) physiological factors that contribute
b) species and breed
c) pathological conditions
11. Leukocytic responses
a) platelets
II. Blood Parasites
A. Babesia
B. Haemobartinella
C. Anaplasmosis
TEST 5 Take home FINAL
Date of revision: January 2003