MEDICAL TECHNOLOGY
PROGRAM
MEDT
462: Medical Microbiology II
3 Credit Hours Spring Semester
INSTRUCTOR: Mark Camblin, Ph.D., SM (ASCP), SM (NRM)
St. Mary’s
Health System Laboratory
Conference
Time: Instructor will be available
for
30 minutes
after class as needed for student conference.
PREREQUISITE: MEDT 461—Medical Microbiology I
COREQUISITE: MEDT 492—Advanced Clinical Practice II
COURSE
DESCRIPTION: MEDT 462 focuses on characteristics of
medically important bacteria, anaerobes, mycobacteria, fungi, viruses, and
parasites of man. Includes principles of
isolation, identification, and antibiotic susceptibility testing. The pathogenesis of organisms and host
response are included also.
TEXTBOOKS:
Company.
Delost, Maria D. (1997).
Introduction to Diagnostic Microbiology: A Text and Workbook.
Company.
METHODS OF
INSTRUCTION: There will be lectures, assigned readings,
case studies, class discussions, and clinical correlations.
COURSE
OBJECTIVES: Upon completion of MEDT 462, the student will
be responsible for attaining the following objectives:
1. Carefully study the assigned chapters in the
textbook, and come to class prepared
to
discuss the topics of the day, as indicated on the Lecture Outline.
2. Apply
results of biochemical tests, gram stain, and culture appearance to identify
the
anaerobic bacteria studied in this class, to include Clostridium, Peptostreptococcus,
Peptococcus, Bacteriodes, Prevotella,
Porphyromonas,
and Veillonella.
3.
Discuss the application of the following stains and be able to interpret
results:
Kinyoun,
Ziehl-Neelsen, Lactophenol Cotton Blue, India Ink, and Trichrome stain.
MEDT 462
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2
4. With
regard to the bacteria and the Mycobacteria
studied in this class, be able to
describe and discuss the following:
Morphology Staining Characteristics
Nomenclature Related Biochemical
Tests
Culture Requirements Related Serological
Tests
Colony Characteristics Special Characteristics
5. Be
able to differentiate among the species of Mycobacteria
by applying results of
biochemical tests and colony appearance.
6. Be
able to identify Nocardia based upon special
stains, colony morphology, and
biochemical
tests.
7. For
each of the following organisms, denote a site in the body where infection
typically
occurs, and describe
the symptoms of an infectious disease produced by each.
Mycoplasma Bacteriodes Veillonella
Chlamydia Clostridia Leptospira
Mycobacteria
Peptococcus Borrelia
Nocardia Peptostreptococcus Treponema
8. For
each group of organisms listed above, select appropriate culture methods, media,
stains
or other mechanisms to help identify and differentiate each.
9. With regard to fungi and yeasts, be able to describe
the following:
a. Germ tube reaction of Candida albicans
b. Dimorphism of Histoplasma, Blastomyces, and Coccidiodes
c. Use of Sabouraud and Mycosel agars, and ingredients
in each.
10. Apply microscopic morphology, colony
appearance, and biochemical tests to identify
the medically important fungi and
yeasts, including Histoplasma, Blastomyces,
Cryptococcus,
Candida, Coccidiodes, and Aspergillus.
11. Be able to differentiate the dermatophytes
from the systemic dimorphic fungi.
12. Recall the scientific name and common name of
each parasite studied in this class.
13. By microscopic morphology, identify the
ameba, ova, larva, and cysts of parasites
that commonly infect humans--as indicated
by the instructor.
14. Correlate parasitic disease with patients’
symptoms and laboratory findings.
15. Discuss the pathophysiology and serologic
diagnosis of viral infection caused by the
medically important viruses, as indicated
by the instructor.
MEDT
462
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3
EVALUATION METHOD:
There will be three
written exams and a comprehensive final exam in this class. A detailed lecture outline with tentative
exam dates is appended at the end of this syllabus. To enhance understanding of the subject
matter, there will be some quizzes and/or homework assignments. The overall grade will be determined as
follows:
3 Written Exams 65
% of
grade
Comprehensive Final Exam 25
% of
grade
Quizzes/ Homework 10
% of
grade
Grading scale is: A = 90
-100%
B+ = 87
- 89 % Medical
Technology students
B = 83
- 86 % must maintain an average of
B-
= 80
- 82 % 80% or higher in order to pass C+
= 77
- 79 % this course.
C = 73
- 76 %
C-
= 70
- 72 %
D = 60
- 69 %
F = below
60 %
Students are strongly
advised to attend all lectures. This
senior-level class meets only once per week, but success in this class requires
the discipline to study throughout the week--not just the night before class.
All students are
expected to take exams as scheduled. If
a student misses an exam due to certifiable illness or a death in the family, a
make-up exam will be offered by appointment only. The format of the make-up exam is at the
instructor’s discretion. The
university’s policy on academic
integrity as stated on page 39 of the LMU Undergraduate Catalog 2003-2004
will be strictly applied in this class.
UNITS OF INSTRUCTION:
Textbook
Lecture 2 Spirochetes,
Chlamydia, Mycoplasma Chapters 20, 21
1-21-04
Lecture 3 Spirochetes,
Chlamydia, Mycoplasma (continued)
Lecture 4 Exam #1 (Chapters 19,
20, 21)
MEDT
462
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4
Textbook
Lecture 6 Mycobacteria
and Nocardia (continued)
Lecture 7 Mycobacteria
and Nocardia Enrichment @ SMMC
Lecture 8 Exam #2 (Chapters 22, 13)
Lecture 9 Mycology Chapter
23
Lecture 10 Mycology
Enrichment @ SMMC
Lecture 11 Exam #3 (Chapter 23)
Lecture 13 Parasitology (continued)
Thursday,
Saturday,