MEDICAL TECHNOLOGY
PROGRAM
MEDT
330: IMMUNOLOGY and SEROLOGY
INSTRUCTOR: Mark Camblin, Ph.D.,
SM (ASCP), SM (NRM)
St. Mary’s
Health System Laboratory
Telephone: 865-545-7649
Conference
Time: Instructor will be available
for 30 minutes
after class
as needed for student conference.
CO-REQUISITE: MEDT 392—Intermediate Clinical Practice II
(Serology
Section)
COURSE
DESCRIPTION: MEDT 330 encompasses a comprehensive study of the human immune
system, including principles of humoral and cellular immunity, autoimmune
responses, defects in the immune system and AIDS. The course also includes theory and
methodology of serology prodedures commonly performed in the clinical
laboratory, with emphasis on serological diagnosis of infectious disease.
TEXTBOOK: Stevens,
Christine D. (2003). Clinical Immunology and
Serology: A Laboratory Perspective, 2nd
Edition.
METHODS OF
INSTRUCTION: Lecture
and Discussion
Assigned
Case
Studies
Clinical
Correlation
COURSE OBJECTIVES: Upon completion of this course, the student
will be responsible for attaining the following objectives:
1.
Describe the basic mechanism of the primary and secondary immune
response.
2.
Discuss the roles of neutrophils, eosinophils, and basophils in the
body’s defense
mechanisms.
3.
Identify three acute phase proteins, and select the most sensitive
serologic test to
detect acute inflammation.
4.
Discuss the composition and function of antigens.
5.
Describe the immunological defects in the following conditions: Chediak-Higashi,
Wiscott-Aldrich, AIDS, DiGeorge’s,
Bruton’s, SCID, and CGD.
6.
Identify the structure and function of each of the five classes of
immunoglobulins.
7.
Discuss the production and use of monoclonal antibodies.
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8.
Explain the function and classification of B-lymphs, T-lymphs, NK- and
K-lymphs.
9.
Describe the activation of
complement by both the classic and alternate pathways.
10. Identify three physiologic effects of
complement activation.
11. Discusss the serologic processes of agglutination (direct
and passive),
precipitation, flocculation, immunodiffusion, and immunofluorescence (direct and indirect); be able name a test that correlates with each
method.
12. Explain
the principle and discuss the application of each of the following methods:
Immunofixation
electrophoresis (
Immunoelectrophoresis Fluorescent-in-situ-Hybridization
(FISH)
ELISA Transcription Mediated
Amplification Fluorescence
Polarization Immunoassay Q-Beta Replicase
Chemluminescent
Immunoassay Ligase Chain Reaction (LCR)
Streptozyme/ASO Polymerase Chain
Reaction (PCR)
Anti-DNase B Lyme EIA and Immunoblot Assays
RPR ANA
VDRL CRP
MHA-TP RA
FTA-ABS Mono
Test
Membrane-Based Cassette Assays Turbidimetry/Nephelometry
13.
Interpret serial dilutions and titers as to diagnostic and therapeutic
implications.
14. Recognize
the quality control testing that is required for serologic procedures, as indicated by the instructor.
15.
Recognize Ouchterlony patterns that characterize partial identity,
nonidentity, and identity.
16.
Discuss the immunological mechanisms of hypersensitivity type I, II,
III, and IV;
give
an example of each.
17.
Discuss the etiology, symptoms, and serologic diagnosis of infectious
diseases caused by the following
organisms:
Hepatitis
A, B, C, D, E Rubella Influenzae A & B Aspergillus
EBV
Rubeola Rotavirus
Coccidioides
CMV Mumps Protozoa/Helminths Candida
Varicella
zoster Treponema Giardia lamblia Blastomyces HIV Borrelia Cryptosporidium Histoplasma
HTLV
I & II Toxoplasma Cryptococcus
18.
Explain one theory for the development of autoimmune disease.
19. Name
five specific autoantibodies, and give a disease association for each.
20.
Compare and contrast the etiology, epidemiology, immunologic
consequences, and
serologic
diagnosis of SLE and RA.
21.
Interpret patterns of fluorescence in ANA testing, and correlate with
possible disease
conditions.
22. Explain
the CDC criteria for the diagnosis of AIDS.
23.
Describe the mechanism of tissue transplant rejection.
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24. Discuss
the use of biologic response modifiers and adoptive immunotherapy in the treatment of cancer.
25. Apply
PCR testing, DNA-probes, and Western blot to the diagnosis of specific diseases.
26. Study
the textbook closely, and come to class prepared to discuss the topics of the day, as indicated on the Lecture Schedule, which is appended at
the end.
EVALUATION METHOD:
There will be three to four written exams and a
comprehensive final exam in this class.
Homework and quizzes will also be given to enhance understanding of
subject material.
The final grade will be based upon:
Written Exams, =
65% of total
grade
Homework/Quizzes = 10% of total grade
Comprehensive
Final Exam = 25% of
total grade
Grading scale: A = 90 -100% Medical
Technology students must
B+ = 87
- 89% maintain
an average of 80% or
B =
83 - 86% above in order to pass this course.
B- =
80 - 82%
C+
= 77
- 79%
C- = 70
- 72%
D = 60
- 69%
F = Below 60%
As upperclassmen,
students are responsible for completing all reading assignments. Students who come
to class, listen, and take notes—plus read the chapters in the textbook—have twice the chance of remembering the
subject material on the board exam. Students are encouraged to ask questions
and participate in class discussions.
Class attendance is expected. If a student is unable to attend class, he or she should notify the professor. The telephone number is listed on the first page of this syllabus. Students are expected to take exams as scheduled. Make-up exams, when justified, will be offered by appointment only. The format of the make-up exam is at the instructor’s discretion. The university’s policy on academic integrity as stated on page 39 of the 2003-2004 LMU Undergraduate Catalog will be strictly applied in this class.
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UNITS OF
INSTRUCTION:
UNIT
I: NATURE OF
THE IMMUNE SYSTEM
A. Historical
Concepts and Introduction to Serological Testing
B.
Natural Immunity
C. The
Lymphoid System
D.
Nature of Antigens and the Major Histocompatibility Complex
E.
Antibody Structure and Function
F. Cytokines
G. Complement
UNIT II: BASIC IMMUNOLOGIC
PROCEDURES
A. Safety and Specimen Preparation
B. Precipitation Reactions
C. Agglutination Reactions
D. Labeled Immunoassays
E. Molecular Biology Techniques
UNIT III: IMMUNE DISORDERS
A. Hypersensitivity
B. Autoimmunity
C. Immunoproliferative Diseases
D. Immunodeficiency Diseases
E. Transplantation Immunology
F. Tumor Immunology
UNIT IV: SEROLOGICAL
DIAGNOSIS OF INFECTIOUS
DISEASES
A. Spirochete Diseases
B. Streptococcal Serology
C. Serology of Viral Infections
D. HIV Serology
E.
Miscellaneous Serology
A Tentative Lecture Schedule with chapter
assignments and exam dates is appended at the end of this syllabus.
DATE OF REVISION: January, 2004
Textbook
Monday
Jan. 19 MARTIN LUTHER KING DAY
Jan. 26 Historical Concepts and Introduction to Serologic Testing
Natural Immunity
The Lymphoid System
Cytokines
Complement
Monday NATURE OF THE IMMUNE SYSTEM Chap.
4, 5
Feb. 2 Nature of Antigens and the Major Histocompatibility Complex
Antibody
Structure and Function
Feb. 9 EXAM #1 (Chapters 1-7)
Agglutination
Reaction
Analysis of Tests
Monday
Mar. 1 EXAM #2 (Chapters 8-12)
Monday IMMUNE DISORDERS Chap. 13,14,15,16,17,18
Mar. 8 Hypersensitivity
Autoimmunity
Immunoproliferative Diseases
Monday IMMUNE DISORDERS Chap. 13,14,15,16,17,18
Mar. 15 Immunodeficiency
Diseases (cont’d)
Transplantation Diseases
Tumor Immunology
Mar. 22 SPRING BREAK
Monday
Mar. 29 EXAM #3 (Chapters 13-18)
Monday SEROLOGIC DIAGNOSIS OF INFECTIOUS DISEASES
Apr. 5 Spirochete
Diseases Chap.
19,20,21,22,23
Streptococcal
Serology
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TENTATIVE LECTURE OUTLINE (cont’d):
Textbook
Monday SEROLOGIC DIAGNOSIS OF INFECTIOUS DISEASES
Apr. 19 SEROLOGIC DIAGNOSIS OF INFECTIOUS DISEASES
HIV
Serology Chap. 19,20,21,22,23
Miscellaneous
Serology (cont’d)
Monday EXAM #4 (Chapters
19-23)
Apr. 26
MONDAY COMPREHENSIVE
FINAL EXAM (Chapters 1-23)
MAY 3
Revised:
MARK CAMBLIN, Ph.D.