MEDICAL TECHNOLOGY PROGRAM
MEDT 302: Introduction to Laboratory Methods and Techniques II
Spring Semester 1 Credit Hour
INSTRUCTOR: Dr. Bill Engle, Th.D., MT (ASCP)
Office: 208 Farr-Chinnock Hall
LMU Telephone: 1-800-325-0900, ext. 6471
Home Telephone: (606) 337-5201
E-mail: bengle@lmunet.edu
Office Hours: Tuesday: 8:00 am to 11:00 am
Thursday:
Instructor will be available for student conference during office hours and also by appointment as needed.
PREREQUISITE: Acceptance in the Medical Technology Program
COURSE DESCRIPTION: MEDT 302 provides a practical introduction to medical microbiology laboratory techniques and builds a foundation for subsequent courses. This course includes specimen collection, microscopic methods, susceptibility testing, laboratory safety, microbiology media, staining techniques, and the basics of the identification of microbes that are commonly encountered in the clinical laboratory.
TEXTBOOK: Delost, Maria D. (1997). Introduction to Diagnostic Microbiology:
A Text and Workbook. St. Louis: C.V. Mosby Company.
METHODS OF INSTRUCTION: Lectures
Demonstrations
Student Laboratory Practice
Assigned
Case
Studies
COURSE OBJECTIVES: Upon completion of MEDT 302, the student will be responsible for attaining the following objectives:
1. Apply elements of laboratory safety in the Microbiology Department.
2. Describe the major routes of infection, and identify the signs and symptoms of
bacterial infection.
3. Follow proper protocol for specimen collection and transport, as outlined in class.
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4. Discuss the uses and composition of the following microbiology media:
Sheep blood MacConkey XLD, SS Campy
blood agar
Chocolate EMB GN
broth V agar
Thayer-Martin Hektoen Thio broth Trypticase
soy broth
5. Select the agar that will support growth of Gram-positive cocci, Gram-negative
bacilli, anaerobes, and fastidious bacteria.
6. Properly streak agar plates to obtain isolated colonies.
7. Describe the colony appearance of the major enterics (as indicated by the instructor)
on selective media.
8. Perform the oxidase test, and discuss its use to differentiate enterics from
non-enterics.
9. Prepare smears from stock cultures and/or clinical specimens.
10. Properly stain and accurately interpret Gram stains.
11. Perform direct methods of examinations, including wet mounts and KOH preps.
11. Define and practice proper skin prep for collection of blood cultures.
13. Discuss the principle and purpose of the catalase test, and be able to interpret results.
and free coagulase,
15. Explain how to differentiate between Staphylococcus and Micrococcus.
16. Describe lab characteristics of the genus Streptococcus, and identify Lancefield
grouping for
beta strep.
17.
Identify biochemical tests that will help you differentiate between Entercoccus and
non-Entercoccus.
18. Be able to differentiate between Streptococcus pneumoniae and viridans
Streptococcus via colony morphology and biochemical tests.
19. Describe
the morphology and biochemical characteristics of Haemophilus.
20.
Explain the consequences of a microorganism being beta-lactamase positive.
21.
Discuss the proper methods of specimen collection and processing for Neisseria
gonorrhea.
22. Identify the microbes that are commonly associated with bacterial meningitis.
23. Describe two modes of microbial resistance to antibiotics.
24. Differentiate between broad-spectrum and narrow-spectrum antibiotics.
25. Demonstrate a micro-broth dilution, and determine the MIC.
26. Interpret Kirby-Bauer susceptibility tests.
27. Compare and contrast the automated identification systems of Vitek and Microscan.
28. Demonstrate proper technique when using the microscopes in student lab; always
remove the oil from the objective when finished, and maintain a neat, clean working
area.
29. Follow instructions of the instructor closely, and be able to accept constructive
criticism, if needed; it is given only to help you.
30. Read the assignment in the textbook before coming to class, and be prepared to
discuss the topics of the day, as indicated on the Tentative Lecture Outline, which
is appended at the end of this syllabus.
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EVALUATION METHOD: The grading scale in this course is as follows:
A = 90-100%
B+ = 87-89%
B = 83-86%
B- = 80-82% Medical Technology
students are required
C+ = 77-79% to maintain an average
of 80% or above
C = 73-76% in order to pass this
course.
C- = 70-72%
D = 60-69%
F = Below
60%
Assignment of the final grade will be based upon the following:
Exam I = 20% of grade
Exam II = 20% of grade
Exam III = 20% of grade
Lab Quizzes = 15% of grade
Comprehensive Final Exam = 25% of grade
Class attendance is expected. Exams and quizzes must be taken on assigned day unless prior arrangements are made with instructor. If a student misses an exam or a quiz, a make-up exam or quiz will be offered, but the format will be at the instructor’s discretion.
Disability Statement: Any student with a disability requiring
special accommodation(s) should make an appointment with the Vice-President for
Student Affairs (LMU extension 6393) to discuss specific needs.
Academic Integrity: It is the aim of the faculty of
UNITS OF INSTRUCTION:
UNIT I: Introduction to the
Hospital Microbiology Department
Biosafety in the Microbiology Lab
Specimen
Collection and Transport
Specimen
Processing
UNIT II: Methods
of Microbiology Examination
Methods
of Identification
Clinical
Specimens
Staphylococcus and Streptococcus
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UNITS OF INSTRUCTION (continued):
UNIT
III: Neisseria
Haemphilus
Enterobacteriaceae
Miscellaneous
Gram Negative Bacteria
Tentative Lecture Schedule:
Date Topic Assigned
Discussion of Syllabus
Discussion of Course Requirements
Safety in the Microbiology Lab Chapter 2
Specimen Transportation
Specimen Processing
Streptococci and Related Organisms Chapter 8
Haemophilus Chapter 12
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5
Tentative Lecture Schedule (continued):
Miscellaneous Gram-neg bacilli Chapter 13
The time and date of the comprehensive exam is to be announced.
DATE OF REVISION: January, 2004